WT Tim-1 moves away from the nascent IS after APC stimulation

Jurkat T cells transiently transfected with Tim-1-GFP (green) and ZAP70-TagRFP (red) were incubated with Raji cells incubated with 1 mg/mL SEE and stained with Cell Tracker Blue (blue). Mid-plane images were taken every 6.7 seconds over the course of 19 minutes in a heated chamber on an Olympus FluoView 1000 confocal microscope.