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Time-lapse imaging of wild-type trypanosomes expressing histone 2B-GFP together with cytosolic GFP or DsRED.

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posted on 2016-04-14, 11:19 authored by Simon Imhof, Cristina Fragoso, Andrew Hemphill, Conrad von Schubert, Dong Li, Wesley R. Legant, Eric Betzig, Isabel Roditi
Images were acquired using a Nikon TE2000E-PFS microscope with a 60x objective. DIC and fluorescent images were taken every 10 minutes for 24 hours.