TAP and LB liquid cultures of the bacterial strain LMJ (Bacterium strain clone LIB091_C05_1243 variant 16S ribosomal RNA; GenBank Accession # MN633292.1) grown for 4 days and Gram stains of LMJ from these liquid cultures. Growth of LMJ on TAP-agar from a 96 hours-grown TAP liquid culture is also shown.

<div>This file contains 11 images. Bacterial strain LMJ was grown in liquid TAP and liquid LB medium for 4 days (96 hours) in an incubator shaker, shaking at 150 rpm at 37C. Images were taken every 24 hours to monitor the turbidity/growth of these liquid cultures over 96 hours. Gram stains were performed on the 24 hours-grown liquid TAP and LB cultures. There were cell debris, membrane fragments, some small rods and round shaped cells were visible after gram staining. <br></div><div>96 hours liquid TAP culture was plated on a TAP-agar medium plate. LMJ grew back within 4 days at room temperature. This result shows that although gram stain could not detect many intact cells in the liquid culture, cells were not dead. LMJ could not form a biofilm in shaking cultures properly and this induced cell lysis in liquid cultures. It can form biofilms on TAP-agar when plated from the liquid TAP culture. </div>