Supplementary data for

dataset

posted on 2015-05-04, 13:57 authored by Anestis Tsakiridis, Val Wilson

Dataset 1 - Figure 1: Raw immunocytochemistry images.
Legend: Fluorescence analysis of TGFP and Sox2 expression in TGFP EpiSCs cultured for 48 hours in FGF2/CHIR following antibody staining against Sox2.

Dataset 2 - Figure 2: Raw immunocytochemistry images.
Legend: Fluorescence analysis and immunocytochemistry of TGFP, Sox2 and Tbx6 expression of in vitro-derived NMPs sorted at day 2 of differentiation and re-plated at high density in the presence of FGF2/CHIR for 2 days.

Dataset 3. Figure 3B - FACS data.
Legend: FACS plots depicting analysis of TGFP expression in day 3 FGF2/CHIR-treated TGFP EpiSCs.

Dataset 4. Figure 3C - Raw immunocytochemistry images.
Legend: Representative examples of the clones obtained after culture of single sorted TGFP+ NMPs in FGF2/CHIR medium following immunofluorescence analysis of T(Bra) and Sox2 expression (Images in figure 3C are magnified parts of raw images).

Dataset 5. Raw data - Tbx6-negative cells.
Legend: Tbx6-negative cells in a clonal population of day 3 TGFP+ NMPs. Clones obtained after culture of single sorted TGFP+ NMPs in FGF2/CHIR medium following immunofluorescence analysis of T(Bra) and Sox2 expression.