DATASET

SIRT1HOS2_Table_1_Blank_reading.csv (1.08 kB)

.CSV

SIRT1HOS2 Table 2 300 ng of rHOS2 protein in absence of trichostatin.csv (1.45 kB)

.CSV

SIRT1HOS2 Table 3 300 ng of rHOS2 protein in presence of trichostatin.csv (1.61 kB)

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3 files

Sirtuin assay of purified Hos2 preparations using resveratrol: UPDATE 1

dataset

posted on 2014-05-27, 07:35 authored by G. Karthikeyan, Maneesh Paul-Satyaseela, Nachiappan Dhatchana Moorthy, Radha Gopalaswamy, Shridhar Narayanan

Fluorimetric Sirtuin1 like deacetylase assay with rHos2 protein using Fluor de Lys®−Sirt1 substrate in presence of Sirtuin1 activator Reserveratrol. This experiment is carried out in presence of HDAC inhibitor Trichostatin to inhibit any residual Histone deacetylase activity. Updated from: http://dx.doi.org/10.6084/m9.figshare.841658.