3 files

Sirtuin assay of purified Hos2 preparations using resveratrol

posted on 12.11.2013, 07:39 authored by G. Karthikeyan, Maneesh Paul-Satyaseela, Nachiappan Dhatchana Moorthy, Radha Gopalaswamy, Shridhar Narayanan

Fluorimetric Sirtuin1 like deacetylase assay with rHos2 protein using Fluor de Lys®−Sirt1 substrate in presence of Sirtuin1 activator Reserveratrol. This experiment is carried out in presence of HDAC inhibitor Trichostatin to inhibit any residual Histone deacetylase activity.