SV2AB and CIP data
ATP hydrolysis reactions were conducted at 25°C with purified recombinant SV2-FLAG or commercially available calf intestinal alkaline phosphatase (CIP). An aliquot of CIP was heated at 95 °C for 20 min to inactivate the enzyme.
At designated intervals (0 min, 1 min, 2 min, 5 min and 10 min), aliquots were removed from the reaction for analysis by the malachite green assay described as above. Duplicates were assayed for each time point and the average value of the duplicates was used for quantification.