Raw data for Figure 5: The presence of IDO and PGE-2 inhibitors reduce the suppression of T cell proliferation by Mesoangioblasts/HIDEMs
Datasets usually provide raw data for analysis. This raw data often comes in spreadsheet form, but can be any collection of data, on which analysis can be performed.
CFSE labelled PBMCs were stimulated with anti CD3/CD28 beads as before in the presence of HIDEMs/mesoangioblasts and inhibitors of IDO and Cox-2, (1-Methyl-L-trypyophan (1MT) (0.5mM) and NS-398 (1.0 uM) respectively, or both. On day 6 cells were harvested and stained with anti-CD3 and 7AAD. Cells were gated on live CD3+ populations and analysed for CFSE dilution and the numbers of cells undergoing CFSE dilution were enumerated using counting beads. Experiments were carried out in duplicates. n=4.