Raw data for Figure 2B: Change of surface marker expression of Mesoangioblasts/HIDEMs upon pro-inflammatory stimulation
datasetposted on 2013-01-25, 12:53 authored by Ou Li, Karen English, Rossana Tonlorenzi, Giulio Cossu, Francesco Saverio Tedesco, Kathryn J. Wood
HIDEMs and mesoangioblasts were stimulated with IFN-γ, TNF-α or IL-1β (20ng/ml) for 24h. Cells were trypsinized and washed, followed by surface staining for HLA-ABC, HLA-DR, CD40, PD-L1 or fluorochrome matched isotype controls and analysis by flow cytometry. Experiments were carried out in duplicates. n=4. Median fluorescence intensities of the markers were examined, and were shown as Mean ± SE.