Raw data for Figure 2B: Change of surface marker expression of Mesoangioblasts/HIDEMs upon pro-inflammatory stimulation
Datasets usually provide raw data for analysis. This raw data often comes in spreadsheet form, but can be any collection of data, on which analysis can be performed.
HIDEMs and mesoangioblasts were stimulated with IFN-γ, TNF-α or IL-1β (20ng/ml) for 24h. Cells were trypsinized and washed, followed by surface staining for HLA-ABC, HLA-DR, CD40, PD-L1 or fluorochrome matched isotype controls and analysis by flow cytometry. Experiments were carried out in duplicates. n=4. Median fluorescence intensities of the markers were examined, and were shown as Mean ± SE.