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Quantitative PCR to verify that VTC1 is a single copy gene in Col-0 and Ler-0 wild types and vtc1-1 and svt2 mutants

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posted on 2013-01-30, 10:48 authored by Chase F. Kempinski, Samuel V. Crowell, Caleb Smeeth, Carina Barth

Quantitative PCR was performed as described in Materials and Methods. The PAD4 gene is a known single copy gene. Therefore, an RQ/RQ ratio of approximately 1 indicates that VTC1 is present in similar quantity as PAD4, and therefore a single-copy gene