Calcein_leak-down_DATA_F1000Res_Dec_2012_edited_go.xlsx (25.67 kB)
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Passive leakage across monolayers using calcein as a fluorescent marker

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posted on 10.05.2013, 14:32 by Steven J Youmans, Miriam R Fein, Elizabeth Wirkowski, John K Maesaka

This dataset tabulates data on passive leakage across the monolayers using a fluorescent marker, calcein, a derivative of fluorescein. The purpose was to gauge the degree of confluence and whether tight junctions had formed between the epithelial cells.

The sheet 'Leak rates' tabulates a summary of the data of the 17 calcein experiments in the raw data sheet. The sheet 'Raw data' contains the data and calculations of the 17 experiments.