Passive leakage across monolayers using calcein as a fluorescent marker
datasetposted on 2013-05-10, 14:32 authored by Steven J Youmans, Miriam R Fein, Elizabeth Wirkowski, John K Maesaka
This dataset tabulates data on passive leakage across the monolayers using a fluorescent marker, calcein, a derivative of fluorescein. The purpose was to gauge the degree of confluence and whether tight junctions had formed between the epithelial cells.
The sheet 'Leak rates' tabulates a summary of the data of the 17 calcein experiments in the raw data sheet. The sheet 'Raw data' contains the data and calculations of the 17 experiments.