Passive leakage across monolayers using calcein as a fluorescent marker
Datasets usually provide raw data for analysis. This raw data often comes in spreadsheet form, but can be any collection of data, on which analysis can be performed.
This dataset tabulates data on passive leakage across the monolayers using a fluorescent marker, calcein, a derivative of fluorescein. The purpose was to gauge the degree of confluence and whether tight junctions had formed between the epithelial cells.
The sheet 'Leak rates' tabulates a summary of the data of the 17 calcein experiments in the raw data sheet. The sheet 'Raw data' contains the data and calculations of the 17 experiments.