Inhibition_pseudotype_virus_entry-1.ods (65.53 kB)
Download file

Inhibition of pseudotype virus entry by existing FDA-approved drugs

Download (0 kB)
posted on 2015-01-29, 12:49 authored by Jason S. Long, Edward Wright, Eleonora Molesti, Nigel TempertonNigel Temperton, Wendy Barclay

293T cells were transfected 24hours previous then mixed with set dilutions of drug and infected with PVs (carried out in triplicate). After 48hours incubation firefly and Renilla values were measured using FLUOstar Omega plate reader (BMG Labtech). Firelfly values were normalised to the corresponding Renilla value. Percent infection at each dilution was calculated using the average values of the untreated control. *values for both ESOM and OM were shared and performed on the same 96-well plate.