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Influence of different histones on neuron cell death and microglial reactivity

posted on 2013-07-08, 11:49 authored by Jonathan D Gilthorpe, Fazal Oozeer, Julia Nash, Margarita Calvo, David LH Bennett, Andrew Lumsden, Adrian Pini

Histone H1 cell death: Images of dissociated embryonic rat cortical neurons exposed to various concentrations of histone 1 (H1) for 48 hours. Some cultures were stained with either propidium iodide (PI - red) or DAPI (blue) after 24 hours to identify dying cells.
Chemotaxis raw data: Images of rat microglia in a Boyden chamber stained with RapiDiffII (blue) at different histone 1 (H1) concentrations. Four independent experiments were performed.
Astrocytes + histones: Images of rat cortical astrocytes exposed to either 0 or 50nM of histone 1 (H1) and anti-glial acidic fibrillary protein (GFAP).
Ox-6 raw data: Images of rat microglia cultured first stained with Iba1 (red), exposed to either 0 or 200nm of histone 1 (H1) for 24 hours before staining with monoclonal mouse anti-rat RT1B antibody (Ox-6; green) to detect MHC-class II. Double-stained cells appear yellow.
Adult cond medium gels: Adult conditioned medium made from rat ischaemic brain slices used to isolate histones.
Adult explants + embryonic: Images of adult rat cortical explants and E16 embryonic rat cortical explants. Beads are made from agarose coated with Sambucus nigra lectin. See Figure 2 in the main text for labeling of images.
Summary raw data sheets: See ‘Legends for summary raw spreadsheets’ in this folder for descriptions of each spreadsheet in this folder.