f1000research.com
Browse
ARCHIVE
Effects of histones on neurons and microglia.zip (111.03 MB)
DOCUMENT
Stats.pdf (205.55 kB)
1/0
2 files

Influence of different histones on neuron cell death and microglial reactivity

dataset
posted on 2013-07-08, 11:49 authored by Jonathan D Gilthorpe, Fazal Oozeer, Julia Nash, Margarita Calvo, David LH Bennett, Andrew Lumsden, Adrian Pini

Histone H1 cell death: Images of dissociated embryonic rat cortical neurons exposed to various concentrations of histone 1 (H1) for 48 hours. Some cultures were stained with either propidium iodide (PI - red) or DAPI (blue) after 24 hours to identify dying cells.
Chemotaxis raw data: Images of rat microglia in a Boyden chamber stained with RapiDiffII (blue) at different histone 1 (H1) concentrations. Four independent experiments were performed.
Astrocytes + histones: Images of rat cortical astrocytes exposed to either 0 or 50nM of histone 1 (H1) and anti-glial acidic fibrillary protein (GFAP).
Ox-6 raw data: Images of rat microglia cultured first stained with Iba1 (red), exposed to either 0 or 200nm of histone 1 (H1) for 24 hours before staining with monoclonal mouse anti-rat RT1B antibody (Ox-6; green) to detect MHC-class II. Double-stained cells appear yellow.
Adult cond medium gels: Adult conditioned medium made from rat ischaemic brain slices used to isolate histones.
Adult explants + embryonic: Images of adult rat cortical explants and E16 embryonic rat cortical explants. Beads are made from agarose coated with Sambucus nigra lectin. See Figure 2 in the main text for labeling of images.
Summary raw data sheets: See ‘Legends for summary raw spreadsheets’ in this folder for descriptions of each spreadsheet in this folder.

 

History

Usage metrics

    f1000research.com

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC