IL-8 Reporter Assay in LPS-stimulated and unstimulated cystic fibrosis airway cells treated with WLBU-2 or LL-37

CF IB3-1 cells (unstimulated and LPS-stimulated) were transfected with a 5' firefly luciferase gene flanking a 200bp wild type- or dNFκB IL-8 promoter and treated with 25µM WLBU-2 or LL-37 followed by measurement of IL-8 promoter activity at time points ranging from 30min-4h by Dual-Luciferase Reporter assay. PBS was used as a control in LPS-stimulated and unstimulated cells. Data represent means/standard deviations of the count readings and calculations of fold-change relative to the PBS-treated controls.