.FCS
Data_1A.fcs (5.65 MB)
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Data_1B.fcs (4.02 MB)
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Data_1C.fcs (4.17 MB)
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Data_1D.fcs (3.3 MB)
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Data_1E.fcs (4.02 MB)
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Data_1F.fcs (4.14 MB)
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Flow cytometry data of spleen cells from wild type, 3H9-transgenic and CD40L/3H9 double transgenic mice
dataset
posted on 2013-10-17, 13:03 authored by Aslam Mohammad, Yusuke Kishi, Takeshi TsubataData 1. Flow cytometry analysis of spleen cells from wild type, 3H9-transgenic and CD40L/3H9 double transgenic mice.
Spleen cells were obtained from wild type (A and D), 3H9-transgenic (B and E), and CD40L/3H9 double transgenic mice (C and F). Cells were stained with Alexa Fluor 647-conjugated (FL 8 channel) rat anti-mouse B220 and Pacific Blue-conjugated (FL 6 channel) anti-mouse Ig λ1(LS-136), and with (D-F) or without (A-C) FITC-conjugated (FL 1 channel) goat anti-mouse Ig λ chain. Lymphoid cells were gated by FSC/SSC dot plots and the B220+ cells were analyzed on a CyAn ADP flow cytometer (Beckman Coulter, USA).
