3 files

Data sets for bacteriophage induction and effect of chitosan

posted on 2014-03-19, 07:59 authored by Leticia Bentancor, Luís C. S. Ferreira, Jaime H. Amorim

(Figure 1A): Induction of TOX:GFP strain by ciprofloxacin and chitosan effect
The induction of TOX:GFP, an E. coli strain carrying a lysogneic 933W mutant bacteriophage in which the stx operon was replaced by a gene encoding GFP, and the effect of chitosan in vitro are shown below. The strain was was induced with ciprofloxacin and the optical density was measured at 600 nm over time (0, 2, 4, 6 hours).

(Figure 1B): Bacteriophage titers
Bacteriophage titers at different conditions are shown over time (hours). Bacteriophage titers were analyzed at 0, 2, 4 and 6 hours post-induction. Chitosan was added at 2 or 4 h post-induction.

(Figure 3B): GFP expression in vivo by IVIS
GFP expression in vivo in mice infected with C600TOX:GFP was measured using In Vivo Imaging System (IVIS). Radiant efficiency in organs of different groups is shown. Four animals per group were analyzed and the fluorescence intensity was quantified using Living Imaging 4.3.1 in Calipter Life Sciences. Each number is an individual mouse. N=5 per group.



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