posted on 2013-03-05, 06:07authored byMargaret E Katz, Katharyn Braunberger, Guancai Yi, Sarah Cooper, Heather M Nonhebel, Cedric Gondro
To monitor autolysis, six flasks containing 50 mL of minimal medium, 10 mM ammonium tartrate and vitamin supplements were each inoculated with 3 x 108 conidia and placed on an orbital shaker. Flasks were removed at 24 or 48 h intervals, the submerged mycelia harvested using Miracloth (Calbiochem/Merck). The dry weights of the mycelia are recorded in this file.