1/1
0/0

Raw data for Figure 2C: HIDEMs and mesoangioblasts fail to induce T cell proliferation in vitro

dataset
posted on 25.01.2013 by Ou Li, Karen English, Rossana Tonlorenzi, Giulio Cossu, Francesco Saverio Tedesco, Kathryn J. Wood

CFSE labelled PBMCs were stimulated with anti CD3/CD28 beads (PBMC+B) as a positive control. HIDEMs and mesoangioblasts were stimulated with IFN-γ, TNF-α or IL-1β (20ng/ml) for 24h. Non-stimulated or cytokine stimulated HIDEMs/mesoangioblasts (ratio 1:4) were then co-cultured with PBMC for 6 days. CD3+ CFSE labelled 7AAD- cells were enumerated using flow cytometry and counting beads. Experiments were carried out in duplicates. n=4.

History

Licence

Exports