10.6084/m9.figshare.94044.v1 Jean Lin Jean Lin Leo Chen Leo Chen Lawrence P Kane Lawrence P Kane WT Tim-1 moves away from the nascent IS after APC stimulation f1000research.com 2012 Tim-1 Cell Tracker Blue (blue) Jurkat T cells confocal microscope Tim-1-GFP Raji cells APC stimulation ZAP70-TagRFP Immunology 2012-08-10 10:47:40 Media https://f1000.figshare.com/articles/media/WT_Tim-1_moves_away_from_the_nascent_IS_after_APC_stimulation_/94044 <p>Jurkat T cells transiently transfected with Tim-1-GFP (green) and ZAP70-TagRFP (red) were incubated with Raji cells incubated with 1 mg/mL SEE and stained with Cell Tracker Blue (blue). Mid-plane images were taken every 6.7 seconds over the course of 19 minutes in a heated chamber on an Olympus FluoView 1000 confocal microscope.</p>