10.6084/m9.figshare.94044.v1
Jean Lin
Jean
Lin
Leo Chen
Leo
Chen
Lawrence P Kane
Lawrence
P Kane
WT Tim-1 moves away from the nascent IS after APC stimulation
f1000research.com
2012
Tim-1
Cell Tracker Blue (blue)
Jurkat T cells
confocal microscope
Tim-1-GFP
Raji cells
APC stimulation
ZAP70-TagRFP
Immunology
2012-08-10 10:47:40
Media
https://f1000.figshare.com/articles/media/WT_Tim-1_moves_away_from_the_nascent_IS_after_APC_stimulation_/94044
<p>Jurkat T cells transiently transfected with Tim-1-GFP (green) and ZAP70-TagRFP (red) were incubated with Raji cells incubated with 1 mg/mL SEE and stained with Cell Tracker Blue (blue). Mid-plane images were taken every 6.7 seconds over the course of 19 minutes in a heated chamber on an Olympus FluoView 1000 confocal microscope.</p>