%0 Generic %A Mohammad, Aslam %A Kishi, Yusuke %A Tsubata, Takeshi %D 2013 %T Flow cytometry data of spleen cells from wild type, 3H9-transgenic and CD40L/3H9 double transgenic mice %U https://f1000.figshare.com/articles/dataset/Flow_cytometry_data_of_spleen_cells_from_wild_type_3H9_transgenic_and_CD40L_3H9_double_transgenic_mice/806234 %R 10.6084/m9.figshare.806234.v1 %2 https://f1000.figshare.com/ndownloader/files/1264302 %2 https://f1000.figshare.com/ndownloader/files/1264300 %2 https://f1000.figshare.com/ndownloader/files/1264301 %2 https://f1000.figshare.com/ndownloader/files/1264303 %2 https://f1000.figshare.com/ndownloader/files/1264304 %2 https://f1000.figshare.com/ndownloader/files/1264305 %K cd40l %K b cells %K auto-immunity %K clonal anergy %K Immunology %X

Data 1. Flow cytometry analysis of spleen cells from wild type, 3H9-transgenic and CD40L/3H9 double transgenic mice.
Spleen cells were obtained from wild type (A and D), 3H9-transgenic (B and E), and CD40L/3H9 double transgenic mice (C and F). Cells were stained with Alexa Fluor 647-conjugated (FL 8 channel) rat anti-mouse B220 and Pacific Blue-conjugated (FL 6 channel) anti-mouse Ig λ1(LS-136), and with (D-F) or without (A-C) FITC-conjugated (FL 1 channel) goat anti-mouse Ig λ chain. Lymphoid cells were gated by FSC/SSC dot plots and the B220+ cells were analyzed on a CyAn ADP flow cytometer (Beckman Coulter, USA).

%I f1000research.com