Data showing positive regulation of Wnt/β-catenin signal transduction by FAM129B siRNA using transcriptional reporter assay, target gene expression, and apoptosis assay William H Conrad Michael B Major Michele A Cleary Marc Ferrer Brian Roberts Shane Marine Namjin Chung William T Arthur Andy J Chien Jason D Berndt Randall T Moon 10.6084/m9.figshare.705153.v1 https://f1000.figshare.com/articles/dataset/Data_showing_postive_regulation_of_Wnt_catenin_signal_transduction_and_Wnt_catenin_by_FAM129B_and_Wnt_dependent_transcriptional_reporter_regulation_by_FAM129B_siRNA/705153 <p>Data for Figure 2b. Measured β-catenin dependent firefly: constitutive renilla ratios for HT1080, A375, and A2058 reporter cells treated with the indicated conditions. Data for Figure 2c: Quantitative RT-PCR of β-catenin dependent Axin2 mRNA and housekeeping actin mRNA for HT1080, A375, and A2058 cells treated with the indicated conditions. Data for Figure 3a. Measured β-catenin dependent firefly: constitutive renilla ratios for A375 reporter cells treated with a dose curve of WNT3A or a single dose of WNT3A. Data for Figure 3b. Measured NFκB dependent firefly: constitutive renilla ratios for A375 reporter cells treated with a dose curve of TNFalpha or a single dose of TNFα . Data for Figure 4b. Quantitation of DAPI and TUNEL positive nuclei from A375 cells treated with WNT3A, PLX4720, and the control, FAM129B, or CTNNB1 siRNA.</p> 2013-06-03 11:34:40 melanoma tumor sirna beta-catenin fibrosarcoma cells FAM129B apopotosis Medicine Molecular Biology