%0 Generic %A Kamel, Suzan %A Yee, John %D 2013 %T Figure 3 raw data: Effect of PTH1R and PKA agonists on high plating density cultures. %U https://f1000.figshare.com/articles/dataset/Figure_3_raw_data_Effect_of_PTH1R_and_PKA_agonists_on_high_plating_density_cultures_/154681 %R 10.6084/m9.figshare.154681.v1 %2 https://f1000.figshare.com/ndownloader/files/479371 %K parathyroid hormone-related protein (PTHrP) %K parathyroid hormone 1 receptor (PTH1R) %K osteoblasts %K intramembranous ossification %K osteoblast colony forming units (CFU-O) %K Physiology %K Cell Biology %K Genetics %X

Fig3A. The amount of cAMP was measured by EIA in 98 wells plated with RC cells and exposed to 0 to 100 nM PTHrP (1-36) for 10 min.
Fig3B. The number of bone nodules in 8 wells following treatment of RC cell cultures with 0 to 1 mM DBcAMP and 0 to 10 µM forskolin (Fsk).
Fig3C. The number of bone nodules in RC cell cultures. RC cells were grown in the absence (control) or presence of 50 nM PTHrP (7-34).

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