%0 Generic %A Li, Ou %A English, Karen %A Tonlorenzi, Rossana %A Cossu, Giulio %A Saverio Tedesco, Francesco %A J. Wood, Kathryn %D 2013 %T Raw data for Figure 5: The presence of IDO and PGE-2 inhibitors reduce the suppression of T cell proliferation by Mesoangioblasts/HIDEMs %U https://f1000.figshare.com/articles/dataset/Raw_data_for_Figure_5_The_presence_of_IDO_and_PGE_2_inhibitors_reduce_the_suppression_of_T_cell_proliferation_by_Mesoangioblasts_HIDEMs/106729 %R 10.6084/m9.figshare.106729.v1 %2 https://f1000.figshare.com/ndownloader/files/238822 %2 https://f1000.figshare.com/ndownloader/files/238855 %2 https://f1000.figshare.com/ndownloader/files/238857 %2 https://f1000.figshare.com/ndownloader/files/238856 %2 https://f1000.figshare.com/ndownloader/files/238859 %2 https://f1000.figshare.com/ndownloader/files/238858 %2 https://f1000.figshare.com/ndownloader/files/238860 %2 https://f1000.figshare.com/ndownloader/files/238861 %K Induced pluripoten stem cells %K Mesangioblasts %K t cells %K Cancer %K Cell Biology %K Immunology %X

CFSE labelled PBMCs were stimulated with anti CD3/CD28 beads as before in the presence of HIDEMs/mesoangioblasts and inhibitors of IDO and Cox-2, (1-Methyl-L-trypyophan (1MT) (0.5mM) and NS-398 (1.0 uM) respectively, or both. On day 6 cells were harvested and stained with anti-CD3 and 7AAD. Cells were gated on live CD3+ populations and analysed for CFSE dilution and the numbers of cells undergoing CFSE dilution were enumerated using counting beads. Experiments were carried out in duplicates. n=4.

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