10.6084/m9.figshare.106727.v1
Ou Li
Ou
Li
Karen English
Karen
English
Rossana Tonlorenzi
Rossana
Tonlorenzi
Giulio Cossu
Giulio
Cossu
Francesco Saverio Tedesco
Francesco
Saverio Tedesco
Kathryn J. Wood
Kathryn
J. Wood
Raw data for Figure 4A: Neutralising antibodies against IFN-γ and TNF-α reduce the immunosuppressive capacity of Mesoangioblasts/HIDEMs
f1000research.com
2013
Induced pluripoten stem cells
Mesangioblasts
t cells
Cancer
Cell Biology
Immunology
2013-01-25 12:53:13
Dataset
https://f1000.figshare.com/articles/dataset/Raw_data_for_Figure_4A_Neutralising_antibodies_against_IFN_and_TNF_reduce_the_immunosuppressive_capacity_of_Mesoangioblasts_HIDEMs/106727
<p>CFSE labelled PBMCs were stimulated with anti-CD3/CD28 beads in the presence of HIDEMs/mesoangioblasts (1:4) and neutralising antibodies against IFN-γ and TNF-α or irrelevant isotype control antibody (0.5, 1.0 and 2.0 µg/ml) or recombinant IL-1RA (0.5, 1.0 and 2.0 µg/ml). Cells were harvested on day 6 and stained with anti-CD3 and 7AAD. After gating on CD3+7AAD- the number of CFSE diluting cells were enumerated using counting beads. Experiments were carried out in duplicates. n=4.</p>