10.6084/m9.figshare.106727.v1 Ou Li Ou Li Karen English Karen English Rossana Tonlorenzi Rossana Tonlorenzi Giulio Cossu Giulio Cossu Francesco Saverio Tedesco Francesco Saverio Tedesco Kathryn J. Wood Kathryn J. Wood Raw data for Figure 4A: Neutralising antibodies against IFN-γ and TNF-α reduce the immunosuppressive capacity of Mesoangioblasts/HIDEMs f1000research.com 2013 Induced pluripoten stem cells Mesangioblasts t cells Cancer Cell Biology Immunology 2013-01-25 12:53:13 Dataset https://f1000.figshare.com/articles/dataset/Raw_data_for_Figure_4A_Neutralising_antibodies_against_IFN_and_TNF_reduce_the_immunosuppressive_capacity_of_Mesoangioblasts_HIDEMs/106727 <p>CFSE labelled PBMCs were stimulated with anti-CD3/CD28 beads in the presence of HIDEMs/mesoangioblasts (1:4) and neutralising antibodies against IFN-γ and TNF-α or irrelevant isotype control antibody (0.5, 1.0 and 2.0 µg/ml) or recombinant IL-1RA (0.5, 1.0 and 2.0 µg/ml). Cells were harvested on day 6 and stained with anti-CD3 and 7AAD. After gating on CD3+7AAD- the number of CFSE diluting cells were enumerated using counting beads. Experiments were carried out in duplicates. n=4.</p>