Raw data for Figure 3C: Mesoangioblasts and HIDEMs do not interfer with T cell activation
Ou Li
Karen English
Rossana Tonlorenzi
Giulio Cossu
Francesco Saverio Tedesco
Kathryn J. Wood
10.6084/m9.figshare.106726.v1
https://f1000.figshare.com/articles/dataset/Raw_data_for_Figure_3C_Mesoangioblasts_and_HIDEMs_do_not_interfer_with_T_cell_activation/106726
<p>CFSE labelled PBMCs (5 x 104/well) were stimulated with anti CD3/CD28 beads (1 x 104/well) (P+B) in the presence or absence of HIDEMs/mesoangioblasts at HIDEM/mesoangioblast:PBMC = 1:4 ratio. Cells were harvested on day 3, 4, 5 or 6 and analysed for CFSE dilution and expression of CD25 and CD69. The number of CD3+7AAD- cells expressing CD25 or CD69 using counting beads and the % of CD25+ and CD69+ cells were calculated from the data. Experiments were carried out in duplicates. n=2.</p>
2013-01-25 12:53:13
Induced pluripoten stem cells
Mesangioblasts
t cells
Cancer
Cell Biology
Immunology