10.6084/m9.figshare.106725.v1 Ou Li Ou Li Karen English Karen English Rossana Tonlorenzi Rossana Tonlorenzi Giulio Cossu Giulio Cossu Francesco Saverio Tedesco Francesco Saverio Tedesco Kathryn J. Wood Kathryn J. Wood Raw data for Figure 3B: Mesoangioblasts and HIDEMs suppress T cell proliferation in a dose dependent manner f1000research.com 2013 Induced pluripoten stem cells Mesangioblasts t cells Cancer Cell Biology Immunology 2013-01-25 12:53:13 Dataset https://f1000.figshare.com/articles/dataset/Raw_data_for_Figure_3B_Mesoangioblasts_and_HIDEMs_suppress_T_cell_proliferation_in_a_dose_dependent_manner/106725 <p>CFSE labelled PBMCs (5 x 104/well) were stimulated with anti CD3/CD28 beads (1 x 104/well) (P+B) in the presence or absence of HIDEMs/mesoangioblasts at decreasing ratios (HIDEM/mesoangioblast:PBMC). On day 6 cells were harvested and stained with anti-CD3 antibody and 7AAD, and analysed by flow cytometry. CFSE dilution was analysed on gated CD3+ 7AAD- cells. The percentage of CD3+CFSE dividing cells was calculated for each group and compared to the positive control (P+B), followed by plotting against HIDEM/mesoangioblast:PBMC ratios. Experiments were carried out in duplicates. n=2</p>